Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH induces HASMC apoptosis. Apoptosis was detected by flow cytometry. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. Percentages of PS-positive/PI-negative and PS-positive/PI-positive cells are shown. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Flow Cytometry, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH increases cleaved caspase 3 and caspase 12 expression as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B, E) Data are the mean ± SD of three independent experiments. (D) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (C, F) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Expressing, Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH increases Bax, Bad, and Bim expression and decreases Bcl-2 expression as detected by RT-PCR. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days.(B) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH activates the PERK-CHOP ER stress pathway as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH activates the IRE1-JNK ER stress pathway as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: PTH increases GRP78/BiP expression as detected by Western blot analysis. (A) HASMCs were treated with 1 × 10 −8 –1 × 10 −6 mol/L PTH for three days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were treated with 1 × 10 −6 mol/L PTH for 0–14 days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus control.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Expressing, Western Blot, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: Inhibition of JNK or CHOP blocks PTH-induced HASMC apoptosis. Apoptosis was detected by flow cytometry. (A) HASMCs were pretreated with SP6000125, a JNK inhibitor, and then treated with 1 × 10 −6 mol/L PTH for 3 days. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were transfected with si-CHOP and then treated with 1 × 10 −6 mol/L PTH for three days. (D) Data are the mean ± SD of three independent experiments. HASMCs cultured in normal medium for the same days as control. * p < .05 versus PTH group.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Inhibition, Flow Cytometry, Transfection, Cell Culture, Control

Journal: Renal Failure

Article Title: Endoplasmic reticulum stress mediates parathyroid hormone-induced apoptosis in vascular smooth muscle cells

doi: 10.1080/0886022X.2022.2027248

Figure Lengend Snippet: Inhibition of JNK or CHOP suppresses PTH-induced cleaved caspase 3 expression as detected by Western blot analysis. (A) HASMCs were pretreated with SP6000125, a JNK inhibitor, and then treated with 1 × 10 −6 mol/L PTH for three days. HASMCs cultured in normal medium for the same days as control. (B) Data are the mean ± SD of three independent experiments. (C) HASMCs were transfected with si-CHOP and then treated with 1 × 10 −6 mol/L PTH for three days. HASMCs cultured in scramble siRNA for the same days as control. (D) Data are the mean ± SD of three independent experiments. * p < .05 versus PTH group.

Article Snippet: Primary human aortic smooth muscle cells (HASMCs) were purchased from ScienCell Research Laboratories (Carlsbad, USA) and cultured as described previously [ ].

Techniques: Inhibition, Expressing, Western Blot, Cell Culture, Control, Transfection

Journal: Neuro-Oncology

Article Title: CRN2 enhances the invasiveness of glioblastoma cells

doi: 10.1093/neuonc/nos388

Figure Lengend Snippet: Involvement of CRN2 in cell proliferation, adhesion, matrix degradation, and invasion. (A) To determine the proliferation rate, 7 × 10 4 cells per cell line were seeded into 60 mm plates at day 0 and counted at day 1 and day 2. For each cell line, the mean proliferation rate between day 1 and day 2 and the standard errors were calculated from 12 measurements derived from 4 independent experiments. (B) Adhesion of the U373 tumor cell lines (1 × 10 5 cells/well of a 96-well plate) to a monolayer of primary human aortic endothelial cells was quantitated using a fluorescence-based protocol. Mean adhesion values and standard errors were calculated from measurements of 16 wells. (C) Matrix degradation in conjunction with the formation of invadopodia was determined using fluorescently labeled gelatin. Invadopodia at the ventral surface of the cells are defined by presence of an F-actin core, matrix degradation by the loss of the TRITC-gelatin signal. The total area of matrix degradation per cell was normalized to the cell area. Mean values per cell line and standard errors were calculated from 30 different cells derived from 3 independent experiments. (D) 0.2 × 10 5 U373 cells per well were seeded on the upper surface of a collagen I–coated semi-porous membrane in a 96-well format. The number of cells that had invaded the collagen matrix and migrated to the lower surface of the membrane (principle of a Boyden chamber) was quantitated by a fluorescence measurement. Mean values and standard errors were calculated from 16 measurements per cell line. *Statistically significant changes, compared with CRN2-shRNA/GFP cells. #Statistically significant changes, compared with CRN2-shRNA/GFP-CRN2-WT cells. See Table 2 for P values.

Article Snippet: Cell adhesion to a confluent monolayer of primary human aortic endothelial cells (Genlantis, PH30405AK) was measured using the CytoSelect Tumor-Endothelium Adhesion Assay (Cell Biolabs, CBA-215) according to the manufacturer's protocol.

Techniques: Derivative Assay, Fluorescence, Labeling, Membrane, shRNA